Plant Tissue Culture

Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book’s experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists.

• Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement
• Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
• Contains the latest advances and updates since the previous edition published in 2012

Table of Contents

1 History of plant cell culture

2 Setup of a tissue culture laboratory

3 Media components and preparation

4 Explant preparation

5 Contamination

6 Callus induction

7 Regeneration and morphogenesis

8 Woody shrubs and trees

9 Haploid plants from anther culture

10 Embryo rescue

11 Meristem culture for virus-free plants

12 In vitro propagation for commercial production of ornamentals

13 Protoplast isolation and fusion

14 Agrobacterium-mediated transformation of plants

15 Genome editing and RNA interference technologies in plants

Appendix I: Useful measurements

Appendix II: Review of solution preparation

Appendix Ill: List of suppliers

Appendix IV: Common plant tissue culture terms

About the Author

Sunghun Park is Professor at the Department of Horticulture and Natural Resources at Kansas State University, USA. He earned his PhD in Plant Physiology from Texas A&M University in 1995 and his main research areas include abiotic stress physiology, crop improvement using plant tissue culture and genome editing technology.